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tryple express enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tryple express enzyme
    Tryple Express Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tryple+express/TrypLE+Express+Enzyme+(1X)/pmc13651307-106-8-14
    Average 99 stars, based on 1 article reviews
    tryple express enzyme - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Passaging:

    Article Title: AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications
    Article Snippet: Wild-type and Suv39h1/Suv39h2/Setdb1/G9a/GLP 5KO iMEFs ( ) were grown in DMEM (Nacalai Tesque) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 1% (v/v) Pen/Strep (Sigma-Aldrich), 1 mM l -glutamine (Sigma-Aldrich), 1% nonessential amino acids (Fujifilm; 100×), 1% 2-mercaptoethanol (Fujifilm; 10 mM stock), and 1% sodium pyruvate (Thermo Fisher Scientific; 100 mM stock) at 37°C in 5% CO 2 . .. Trypsinization for cell passaging was performed using TrypLE Express (Thermo Fisher Scientific, 12605010) or 0.25% trypsin/0.02% EDTA in phosphate-buffered saline (PBS; Nacalai Tesque). .. The day before imaging, cells were seeded on 35-mm MatTek chambers or 24-well glass-bottom plates (AGC Techno Glass) at ~70% confluency and transfected.

    Article Title: AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.
    Article Snippet: Wild- type and Suv39h1/Suv39h2/Setdb1/G9a/GLP 5KO iMEFs (75) were grown in DMEM (Nacalai Tesque) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 1% (v/v) Pen/Strep (Sigma- Aldrich), 1 mM l- glutamine (Sigma- Aldrich), 1% nonessential amino acids (Fujifilm; 100×), 1% 2- mercaptoethanol (Fujifilm; 10 mM stock), and 1% sodium pyruvate (Thermo Fisher Scientific; 100 mM stock) at 37°C in 5% CO2. .. Trypsinization for cell passaging was performed using TrypLE Express (Thermo Fisher Scientific, 12605010) or 0.25% trypsin/0.02% EDTA in phosphate- buffered saline (PBS; Nacalai Tesque). .. The day before imaging, cells were seeded on 35- mm MatTek chambers or 24- well glass- bottom plates (AGC Techno Glass) at ~70% confluency and transfected.

    Article Title: A Human Neuronal Co-Culture System Reveals Early Tumor-Neuron Communication and Targetable Pathways in Glioblastoma
    Article Snippet: .. For passaging, NU-757 cells were dissociated into single-cell suspensions using TrypLE Express (phenol red–free; Gibco, Cat. #12604013). .. Human induced pluripotent stem cells (iPSCs) of the KOLF2.1 line (Jackson Laboratory for Genomic Medicine) were cultured in mTeSRTM Plus medium (STEMCELL Technologies) on Matrigel-coated 6-well plates (BD MatrigelTM hESC-qualified Matrix, Cat. #354277; coating time for 30 minutes at room temperature).

    Saline:

    Article Title: AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications
    Article Snippet: Wild-type and Suv39h1/Suv39h2/Setdb1/G9a/GLP 5KO iMEFs ( ) were grown in DMEM (Nacalai Tesque) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 1% (v/v) Pen/Strep (Sigma-Aldrich), 1 mM l -glutamine (Sigma-Aldrich), 1% nonessential amino acids (Fujifilm; 100×), 1% 2-mercaptoethanol (Fujifilm; 10 mM stock), and 1% sodium pyruvate (Thermo Fisher Scientific; 100 mM stock) at 37°C in 5% CO 2 . .. Trypsinization for cell passaging was performed using TrypLE Express (Thermo Fisher Scientific, 12605010) or 0.25% trypsin/0.02% EDTA in phosphate-buffered saline (PBS; Nacalai Tesque). .. The day before imaging, cells were seeded on 35-mm MatTek chambers or 24-well glass-bottom plates (AGC Techno Glass) at ~70% confluency and transfected.

    Article Title: AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.
    Article Snippet: Wild- type and Suv39h1/Suv39h2/Setdb1/G9a/GLP 5KO iMEFs (75) were grown in DMEM (Nacalai Tesque) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 1% (v/v) Pen/Strep (Sigma- Aldrich), 1 mM l- glutamine (Sigma- Aldrich), 1% nonessential amino acids (Fujifilm; 100×), 1% 2- mercaptoethanol (Fujifilm; 10 mM stock), and 1% sodium pyruvate (Thermo Fisher Scientific; 100 mM stock) at 37°C in 5% CO2. .. Trypsinization for cell passaging was performed using TrypLE Express (Thermo Fisher Scientific, 12605010) or 0.25% trypsin/0.02% EDTA in phosphate- buffered saline (PBS; Nacalai Tesque). .. The day before imaging, cells were seeded on 35- mm MatTek chambers or 24- well glass- bottom plates (AGC Techno Glass) at ~70% confluency and transfected.

    Single Cell:

    Article Title: Mechanisms inducing differentiation of adult islet progenitor-like cells into functional islet-like organoids
    Article Snippet: .. Cells were dissociated into single-cell suspensions using TrypLE Express (Gibco, no phenol red) at 37°C for 5–8 min and diluted 1:1 with PBS. ..

    Article Title: A Human Neuronal Co-Culture System Reveals Early Tumor-Neuron Communication and Targetable Pathways in Glioblastoma
    Article Snippet: .. For passaging, NU-757 cells were dissociated into single-cell suspensions using TrypLE Express (phenol red–free; Gibco, Cat. #12604013). .. Human induced pluripotent stem cells (iPSCs) of the KOLF2.1 line (Jackson Laboratory for Genomic Medicine) were cultured in mTeSRTM Plus medium (STEMCELL Technologies) on Matrigel-coated 6-well plates (BD MatrigelTM hESC-qualified Matrix, Cat. #354277; coating time for 30 minutes at room temperature).

    Article Title: 10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models.
    Article Snippet: Sample preparation: fixation and storage PC9 cells were grown in RPMI 1640 with Glutamax (Gibco) and 10% FBS, while NIH-3T3 cells were grown in DMEM (Gibco) with L-glutamine and 10% FBS. .. Cells were dissociated using Tryple Express (no phenol red) (Gibco) and made into single-cell suspension, followed by filtration through 30uM Miltenyi pre-separation filters and counting using AO/PI double stain (Nexcelom). ..

    Incubation:

    Article Title: Exploring extracellular vesicle MicroRNAs in Usher syndrome type 1B: Tear-Derived EVs as potential indicators of retinal health.
    Article Snippet: .. For passing, hiPSC-RPE cells were first washed with 1x PBS and then incubated twice with TrypLe Express (no phenol red, Gibco) during 10 minutes at 37°C to detach those cells not differentiated to RPE. ..

    Suspension:

    Article Title: 10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models.
    Article Snippet: Sample preparation: fixation and storage PC9 cells were grown in RPMI 1640 with Glutamax (Gibco) and 10% FBS, while NIH-3T3 cells were grown in DMEM (Gibco) with L-glutamine and 10% FBS. .. Cells were dissociated using Tryple Express (no phenol red) (Gibco) and made into single-cell suspension, followed by filtration through 30uM Miltenyi pre-separation filters and counting using AO/PI double stain (Nexcelom). ..

    Filtration:

    Article Title: 10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models.
    Article Snippet: Sample preparation: fixation and storage PC9 cells were grown in RPMI 1640 with Glutamax (Gibco) and 10% FBS, while NIH-3T3 cells were grown in DMEM (Gibco) with L-glutamine and 10% FBS. .. Cells were dissociated using Tryple Express (no phenol red) (Gibco) and made into single-cell suspension, followed by filtration through 30uM Miltenyi pre-separation filters and counting using AO/PI double stain (Nexcelom). ..

    Staining:

    Article Title: 10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models.
    Article Snippet: Sample preparation: fixation and storage PC9 cells were grown in RPMI 1640 with Glutamax (Gibco) and 10% FBS, while NIH-3T3 cells were grown in DMEM (Gibco) with L-glutamine and 10% FBS. .. Cells were dissociated using Tryple Express (no phenol red) (Gibco) and made into single-cell suspension, followed by filtration through 30uM Miltenyi pre-separation filters and counting using AO/PI double stain (Nexcelom). ..



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